Description
GI360 Complete is an advanced, innovative, comprehensive and clinically-applicable stool profile, utilising multiplex PCR molecular technology, coupled with growth-based culture and ID by MALDI-TOF (>1200 bacteria; >200 yeasts can be identified), sensitive biochemical assays and microscopy to detect and assess the status of pathogens, viruses, parasites and bacteria that may be contributing to acute of chronic gastrointestinal symptoms and disease. GI360 Complete includes a broad range of stool chemistry markers to assess digestion/absorption, inflammation, immunology, short chain fatty acids and intestinal health markers including beta-glucuronidase.
Also includes zonulin and H pylori:
Elevated faecal zonulin family protein has been associated with increased intestinal permeability, metabolic syndrome and obesity.
Helicobacter Pylori is a microorganism which can be found on the stomach mucosa and may contribute to gastritis, gastric ulcers, GERD and other serious pathologies.
Methodology
The test is broken down into sections:
Commensal bacteria and GI Pathogens measured by multiplex PCR.
Gold standard microscopy of parasites, worms and yeasts.
Microscopy of red and white blood cells, muscle fibres, vegetable fibres, pollen and Charcot-Leyden Crystals.
Culturing of commensal and pathogenic bacteria
Macroscopic assessment of stool colour, consistency and presence/ absence of mucus.
Elastase and Calprotectin - BÜHLMANN fCAL® & fPELA® turbo assays
H Pylori, Lactoferrin, Lysozyme, sIgA, Zonulin - Elisa
Carbohydrates – Colormetric
Fat Stain – Sudan IV
SCFA – Gas Chromatography
Beta Glucuronidase - Quantitative endpoint enzymatic (RUO)
Occult Blood – Guaiac
Stool pH – ISE
When should I use
The GI360 Complete with zonulin and H. Pylori can help to differentiate inflammation associated with inflammatory bowel disease (IBD) from that noted in irritable bowel syndrome (IBS). The test can be used to identify underlying factors in digestive symptoms. The dysbiosis index, based on PCR assessment of commensal bacteria, was devised using the ROME criteria for IBS and bacteria associated with IBD. This profile is indicated for gastrointestinal symptoms, autoimmune disease, gastritis, gastric ulcers, GERD, joint pain, IBD, IBS, inflammation, increased gut permeability, food sensitivities, nutritional deficiencies and skin conditions.
Other Details
If zonulin and H Pylori are not required, consider DD003 GI360 Complete.
